Determining the Composition of Lignins in Different Tissues of Silver Birch
Lignin is a complex macromolecule, and hence, it has been stated many times that there is no single method for the accurate determination of the quality or quantity of lignin [ 8 , 25 ]. Because of the high molecular weight and insolubility, knowledge of the composition of wood lignin is obtained indirectly, e.
This can be done with CuO and thioacidolysis methods, which give valuable information on the monolignol composition of lignin. Downscaling of the sample size presents another problem: representative analysis and results are challenging if the sample size is on a mg scale as, e. This is more accentuated in samples containing other tissues than xylem as, e. Similar results have been found in poplar tissues by thioacidolysis [ 19 ].
The project was funded by a grant from the Academy of Finland Grant No.
We wish to thank our technician, Eija Rinne, for the cryotome sectioning of the silver birch woody material, and Irmeli Luovula and Hanna Kautto for the lignin analyses. National Center for Biotechnology Information , U. Journal List Plants Basel v. Plants Basel. Published online Apr 9. Kurt V. Find articles by Kurt V. Find articles by Tarja Tapanila. Find articles by Juan Antonio Alonso Serra. Find articles by Kaisa Nieminen. Philip Harris, Academic Editor. Author information Article notes Copyright and License information Disclaimer. Received Dec 5; Accepted Mar This article has been cited by other articles in PMC.
Abstract Quantitative and qualitative lignin analyses were carried out on material from the trunks of silver birch Betula pendula Roth trees. Keywords: acetyl bromide, Betula pendula , cupric oxide, lignin analysis methods, phloem, thioacidolysis, xylem. Introduction Lignin is an aromatic, heterogenic cell wall heteropolymer that is essential for mechanical support, water transport and disease resistance in trees [ 1 ].
Results and Discussion 2. Lignin Composition of Different Silver Birch Tissue Fractions Different tissues were cryosectioned from year-old whole silver birch stems and divided into five fractions Figure 1. Open in a separate window. Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. Figure 6. Figure 7. Experimental Section For the different tissues of the silver birch trunk Betula pendula Roth , we felled three birch trees at the experimental field of the Viikki Campus at the University of Helsinki.
Quantitative Analysis of Lignin 3. Qualitative Analysis of Lignin 3. Conclusions Lignin is a complex macromolecule, and hence, it has been stated many times that there is no single method for the accurate determination of the quality or quantity of lignin [ 8 , 25 ]. Author Contributions K. Conflicts of Interest The authors declare no conflict of interest. References 1.
Ralph J. Encyclopedia of Life Sciences. Vanholme R. Metabolic engineering of novel lignin in biomass crops. New Phytol. Eudes A. Chiang V. Comparison of softwood and hardwood kraft pulping. Pulp Pap. Dence C. The determination of lignin. In: Lin S.
Methods in Lignin Chemistry
Methods in Lignin Chemistry. Springer-Verlag; Heidelberg, Germany: Downscaled method using glass microfiber filters for the determination of Klason lignin and structural carbohydrates. Biomass Bioenergy. Hatfield R. Can lignin be accurately measured? Crop Sci. Brinkmann K. Comparison of different methods for lignin determination as a basis for calibration of near-infrared reflectance spectroscopy and implications of lignoproteins.
Moreira-Vilar F. The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than klason and thioglycolic acid methods. Johnson D. The spectrophotometric determination of lignin in small wood samples. Lapierre C. Preparative thioacidolysis of spruce lignin: Isolation and identification of main monomeric products. Determining lignin structure by chemical degradations. In: Heitner C. Lignin and Lignans. Advances in Chemistry. Thevenot M.
Fate of lignins in soils: A review.
Collecting Books on NYC
Soil Biol. Spielvogel S. Changes of lignin phenols and neutral sugars in different soil types of a high-elevation forest ecosystem 25 years after forest dieback. Kuo L. Fate of CuO-derived oxidation products during plant combustion: Application to the evaluation of char input to soil organic matter. Hedges J. Characterization of lignin by capillary gas chromatography of cupric oxide oxidation products.
Kaiser K. Characterization of lignin by gas chromatography and mass spectrometry using a simplified CuO oxidation method. Hydroxycinnamates in lignification. Christiernin M. Lignin composition in cambial tissues of poplar. Plant Physiol. Yue F. Synthesis of lignin-derived thioacidolysis monomers and their uses as quatitation standards.
- Principles and Practices of Lighting Design: The Art of Lighting Composition.
- Methods in lignin chemistry ;
- ISBN 10: 3642740677;
- Methods in lignin chemistry ( edition) | Open Library.
- Lady Alexandras Excellent Adventure: A Summersby Tale (Summersby Tales).
Food Chem. KCL; Espoo, Finland: Massan ja Puun Kokonaisligniinipitoisuus. Total lignin content of wood and pulp p.
Methods in lignin chemistry
Using the acetyl bromide assay to determine lignin concentrations in herbaceous plants: Some cautionary notes. Rolando C. Springer-Verlag; Berlin, Germany: Springer Series in Wood Science. Alkaline CuO oxidation with a microwave digestion system: Lignin analyses of geochemical samples. Davin L. Lignins: A twenty-first century challenge. In: Himmel M.
- Special order items;
- Bob Marley: Lyrical Genius.
- Methods in Lignin Chemistry | Stephen Y. Lin | Springer.
- Wranglers Lady (A Love & Laughter Romance).
- Methods in Lignin Chemistry (Springer Series in Wood Science).
Biomass Recaltritrance. Deconstructing the Plant Cell Wall for Bioenergy. Blackwell Publishing Ltd. Save to Library. Create Alert. Figures, Tables, and Topics from this paper. Figures and Tables. Wiley Fragmentation computing. Citations Publications citing this paper. Laccases and their applications: a patent review. Methods in Lignin Chemistry Stephen Y. Lin , Carlton W. References Publications referenced by this paper. Gaschromatografische Analyse yon Ligninoxy. Erickson , S. Larsson , G. Part 5. Gellerstedt , Kristina Gustafsson.
Gellerstedt , Eva-Lisa Lindfors.